Chapter: Expression of Antibodies in Mammalian Cells

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Home > Antibody Engineering > Expression of Antibodies in Mammalian Cells

Antibody Engineering |

Roland E. Kontermann; Stefan Dübel

Springer Berlin Heidelberg

Expression of Antibodies in Mammalian Cells

Chapter Info

Authors

Andrew Bradbury

ARI Id

1664316757244_1183395

Access

Not Available Free

Pages

357-366

DOI

10.1007/978-3-662-04605-0_26

Chapter URL

https://rd.springer.com/chapter/10.1007/978-3-662-04605-0_26

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Chapters/Headings	Author(s)	Pages	Info
Front Matter		I-XII
Front Matter		1-1
Recombinant Antibodies	Stefan Dübel; Roland E. Kontermann	3-16
Front Matter		17-17
Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly	Jörg Burmester; Andreas Plückthun	19-40
Construction of scFv from Hybridoma by Two-Step Cloning	Frank Breitling; Dieter Moosmayer; Bodo Brocks; Stefan Dübel	41-55
Cloning Hybridoma cDNA by RACE	Andrew Bradbury	56-61
Front Matter		63-63
Phage — Display Libraries of Murine Antibody Fab Fragments	Jan Engberg; Liselotte Brix Jensen; Ali Fazil Yenidunya; Katja Brandt; Erik Riise	65-92
Generation of Naive Human Antibody Libraries	Catherine Hutchings; Sara Carmen; Simon Lennard	93-108
Generation of Antibody Gene Libraries from Seropositive Human Donors	Joachim Koch; Stefan Dübel	109-123
Generation of Rabbit Immune Libraries	Rüdiger Ridder; Hermann Gram	124-134
Front Matter		135-135
Immunotube Selections	Roland E. Kontermann	137-148
Selections on Biotinylated Antigens	Patrick Chames; Hennie R. Hoogenboom; Paula Henderikx	149-166
Guided Selection on Cells	Silvana Canevari; Mariangela Figini	167-183
Proximity (ProxiMol) and Step-Back Selections	Jane K. Osbourn	184-192
Selections on Tissue Sections	Jesper Max Tordsson; Thomas Niklas Brodin; Pia Jasmine Karlström	193-205
Selection of Human Antibody Fragments by Anti-Idiotypic Interaction	Peter Fischer	206-212
Selecting Intracellular Antibodies Using the Two-Hybrid System	Michela Visintin; Antonino Cattaneo	213-233
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