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Home > JAK-STAT Signalling: Methods and Protocols > Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins
JAK-STAT Signalling: Methods and Protocols |
Sandra E. Nicholson; Nicos A. Nicola
Humana Press
JAK-STAT Signalling: Methods and Protocols
Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins
Authors

Florence Baudin; Christoph W. Müller

ARI Id

1664540366984_2118628

Access

Not Available Free

Pages

301-317

DOI

10.1007/978-1-62703-242-1_21

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-62703-242-1_21

Table of Contents of Book
Showing 1 to 20 of 27 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xi
Front Matter
1-1
Analysis of Janus Tyrosine Kinase Phosphorylation and Activation
Jeremy A. Ross; Georgialina Rodriguez; Robert A. Kirken
3-20
Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e. g. , Janus Kinases or Other Associated Signaling Proteins
Claude Haan; Serge Haan
21-38
In Vitro JAK Kinase Activity and Inhibition Assays
Jeffrey J. Babon; James M. Murphy
39-55
Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Resonance
Jeffrey J. Babon
57-67
Determination of Protein Turnover Rates in the JAK/STAT Pathway Using a Radioactive Pulse-Chase Approach
Anna Dittrich; Elmar Siewert; Fred Schaper
69-80
Designing RNAi Screens to Identify JAK/STAT Pathway Components
Katherine H. Fisher; Stephen Brown; Martin P. Zeidler
81-97
The Use of JAK-Specific Inhibitors as Chemical Biology Tools
Christopher J. Burns; David Segal; Andrew F. Wilks
99-113
Methods for Detecting Mutations in the Human JAK2 Gene
Anthony J. Bench; E. Joanna Baxter; Anthony R. Green
115-131
Front Matter
133-133
Detection of Activated STAT Proteins
Jean-Patrick Parisien; Curt M. Horvath
135-145
Detection of Activated STAT Species Using Electrophoretic Mobility Shift Assay (EMSA) and Potential Pitfalls Arising from the Use of Detergents
Serge Haan; Claude Haan
147-159
Flow Cytometric Analysis of STAT Phosphorylation
Jane Murphy; Gabrielle L. Goldberg
161-165
Acetylation of Endogenous STAT Proteins
Torsten Ginter; Thorsten Heinzel; Oliver H. Krämer
167-178
Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining
Reza Khorooshi; Trevor Owens
179-188
Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging
Velasco Cimica; Nancy C. Reich
189-202
Characterization of STAT Self-Association by Analytical Ultracentrifugation
Nikola Wenta; Uwe Vinkemeier
203-224
Constitutively Active STAT5 Constructs
Lynn M. Heltemes-Harris; Michael A. Farrar
225-232
Front Matter
233-233
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 27 entries
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