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Home > Heterologous Expression of Membrane Proteins: Methods and Protocols > Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization
Heterologous Expression of Membrane Proteins: Methods and Protocols |
Isabelle Mus-Veteau
Springer New York
Heterologous Expression of Membrane Proteins: Methods and Protocols
Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization
Authors

Jennifer L. Johnson; Sibel Kalyoncu; Raquel L. Lieberman

ARI Id

1664541797499_2123671

Access

Not Available Free

Pages

281-301

DOI

10.1007/978-1-4939-3637-3_18

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-4939-3637-3_18

Table of Contents of Book
Showing 1 to 20 of 21 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xii
Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies
Ralf-Bernhardt Rues; Erik Henrich; Coilin Boland; Martin Caffrey; Frank Bernhard
1-21
Membrane Protein Production in the Yeast, S. cerevisiae
Stephanie P. Cartwright; Lina Mikaliunaite; Roslyn M. Bill
23-35
Membrane Protein Production in Escherichia coli: Protocols and Rules
Federica Angius; Oana Ilioaia; Marc Uzan; Bruno Miroux
37-52
Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach
Kiavash Mirzadeh; Stephen Toddo; Morten H. H. Nørholm; Daniel O. Daley
53-61
Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins
Juni Andréll; Patricia C. Edwards; Fan Zhang; Maria Daly; Christopher G. Tate
63-78
Membrane Protein Production in Lactococcus lactis for Functional Studies
Daphne Seigneurin-Berny; Martin S. King; Emiline Sautron; Lucas Moyet; Patrice Catty; François André et al.
79-101
Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells
Renato Mancini Astray; Sandra Fernanda Suárez-Patiño; Soraia Attie Calil Jorge
103-118
Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6
Lucia Gonzalez-Lobato; Vincent Chaptal; Jennifer Molle; Pierre Falson
119-131
Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA
Benjamin Wiseman; Jean-Michel Jault
133-142
Expression of Eukaryotic Membrane Proteins in Pichia pastoris
Lucie Hartmann; Valérie Kugler; Renaud Wagner
143-162
Integral Membrane Protein Expression in Saccharomyces cerevisiae
Rebba C. Boswell-Casteel; Jennifer M. Johnson; Robert M. Stroud; Franklin A. Hays
163-186
High-Throughput Baculovirus Expression System for Membrane Protein Production
Ravi C. Kalathur; Marinela Panganiban; Renato Bruni
187-202
Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies
Sarika Chaudhary; Sukanya Saha; Sobrahani Thamminana; Robert M. Stroud
203-221
Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies
Rajendra Boggavarapu; Stephan Hirschi; Daniel Harder; Marcel Meury; Zöhre Ucurum; Marc J. Bergeron et al.
223-242
Membrane Protein Solubilization and Composition of Protein Detergent Complexes
Katia Duquesne; Valérie Prima; James N. Sturgis
243-260
Detergent-Free Membrane Protein Purification
Alice J. Rothnie
261-267
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Eric Forest; Petr Man
269-279
Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization
Jennifer L. Johnson; Sibel Kalyoncu; Raquel L. Lieberman
281-301
Method to Screen Multidrug Transport Inhibitors Using Yeast Overexpressing a Human MDR Transporter
Laura Fiorini; Isabelle Mus-Veteau
303-318
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 21 entries
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