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Home > Protocols for Nucleic Acid Analysis by Nonradioactive Probes > Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR
Protocols for Nucleic Acid Analysis by Nonradioactive Probes |
Elena Hilario; John F. MacKay
Humana Press
Protocols for Nucleic Acid Analysis by Nonradioactive Probes
Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR
Authors

Sukru Tuzmen; Jeff Kiefer; Spyro Mousses

ARI Id

1664586376794_2308324

Access

Not Available Free

Pages

177-203

DOI

10.1385/1-59745-229-7:177

Chapter URL

https://rd.springer.com/protocol/10.1385/1-59745-229-7:177

Table of Contents of Book
Showing 1 to 20 of 26 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-x
Front Matter
1-1
Genomic DNA Isolation From Different Biological Materials
Duckchul Park
3-13
Extraction of Plant RNA
Elspeth MacRae
15-24
Front Matter
25-25
Overview of Hybridization and Detection Techniques
Elena Hilario
27-38
Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection
Lisa S. Gellen; Glenn M. Wall-Manning; Chris H. Sissons
39-67
Nonradioactive Northern and Southern Analyses From Plant Samples
Christoph Peterhaensel; Dagmar Weier; Thomas Lahaye
69-78
Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes
Elena Hilario; Tiffany F. Bennell; Erik Rikkerink
79-91
Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes
Saeed A. Khan; Mohamed S. Nawaz
93-103
In Situ Hybridization of Termite Microbes
Shigeharu Moriya; Satoko Noda; Moriya Ohkuma; Toshiaki Kudo
105-112
Front Matter
113-113
RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer
Yukinori Eguchi
115-124
Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization
Darren J. Day; Eli M. Mrkusich; John H. Miller
125-142
Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification
María Rosa Ponce; Víctor Quesada; Andrea Hricová; José Luis Micol
143-152
Fluorescence In Situ Hybridization for the Identification of Environmental Microbes
Annelie Pernthaler; Jakob Pernthaler
153-164
Front Matter
165-165
Introduction to Kinetic (Real-Time) PCR
John Mackay
167-176
Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR
Sukru Tuzmen; Jeff Kiefer; Spyro Mousses
177-203
Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes
Jasmien Hoebeeck; Frank Speleman; Jo Vandesompele
205-226
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 26 entries
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