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Home > Protein Chromatography: Methods and Protocols > Ion-Exchange Chromatography: Basic Principles and Application
Protein Chromatography: Methods and Protocols |
Dermot Walls; Sinéad T. Loughran
Springer New York
Protein Chromatography: Methods and Protocols
Ion-Exchange Chromatography: Basic Principles and Application
Authors

Philip M. Cummins; Keith D. Rochfort; Brendan F. O’Connor

ARI Id

1664683967494_2716786

Access

Not Available Free

Pages

209-223

DOI

10.1007/978-1-4939-6412-3_11

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-4939-6412-3_11

Table of Contents of Book
Showing 1 to 20 of 27 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-x
Front Matter
1-1
A Synopsis of Proteins and Their Purification
Dermot Walls; Gary Cooney; Sinéad T. Loughran
3-14
Gel-Filtration Chromatography
Ciarán Ó’Fágáin; Philip M. Cummins; Brendan F. O’Connor
15-25
Immunoaffinity Chromatography: Concepts and Applications
Jenny Fitzgerald; Paul Leonard; Elaine Darcy; Shikha Sharma; Richard O’Kennedy
27-51
Avoiding Proteolysis During Protein Purification
Barry J. Ryan; Gary T. Henehan
53-69
Scale-Up of Protein Purification: Downstream Processing Issues
John Joseph Milne
71-84
Phage Display: A Powerful Technology for the Generation of High-Specificity Affinity Reagents from Alternative Immune Sources
William J. J. Finlay; Laird Bloom; Joanne Grant; Edward Franklin; Deirdre Ní Shúilleabháin; Orla Cunningham
85-99
Protein Stability: Enhancement and Measurement
Ciarán Ó’Fágáin
101-129
Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
Sinéad T. Loughran; Dermot Walls
131-156
Front Matter
157-157
Storage and Lyophilization of Pure Proteins
Ciarán Ó’Fágáin; Keith Colliton
159-190
Differential Precipitation and Solubilization of Proteins
Barry J. Ryan; Gemma K. Kinsella
191-208
Ion-Exchange Chromatography: Basic Principles and Application
Philip M. Cummins; Keith D. Rochfort; Brendan F. O’Connor
209-223
Protein Quantitation and Analysis of Purity
Eva M. Campion; Sinéad T. Loughran; Dermot Walls
225-255
Purification of Proteins Fused to Maltose-Binding Protein
Mario Lebendiker; Tsafi Danieli
257-273
Purification of Polyhistidine-Tagged Proteins
Sinéad T. Loughran; Ronan T. Bree; Dermot Walls
275-303
Purification of Antibodies Using Affinity Chromatography
Elaine Darcy; Paul Leonard; Jenny Fitzgerald; Martin Danaher; Hui Ma; Richard O’Kennedy
305-318
Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
William J. J. Finlay; Laird Bloom; Sreeja Varghese; Bénédicte Autin; Orla Cunningham
319-338
Measuring Protein–Protein Interactions Using Biacore
Paul Leonard; Stephen Hearty; Hui Ma; Richard O’Kennedy
339-354
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 27 entries
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