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Home > Rational Drug Design: Methods and Protocols > Using a Modified Yeast Two-Hybrid System to Screen for Chemical GEF Inhibitors
Rational Drug Design: Methods and Protocols |
Yi Zheng
Humana Press
Rational Drug Design: Methods and Protocols
Using a Modified Yeast Two-Hybrid System to Screen for Chemical GEF Inhibitors
Authors

Anne Blangy; Philippe Fort

ARI Id

1665059186134_4653946

Access

Not Available Free

Pages

81-95

DOI

10.1007/978-1-62703-008-3_7

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-62703-008-3_7

Table of Contents of Book
Showing 1 to 19 of 19 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xi
On Setting Up and Assessing Docking Simulations for Virtual Screening
Jacek Biesiada; Aleksey Porollo; Jaroslaw Meller
1-16
Virtual Ligand Screening Combined with NMR to Identify Dvl PDZ Domain Inhibitors Targeting the Wnt Signaling
Jufang Shan; Jie J. Zheng
17-28
Rational Design of Rho GTPase-Targeting Inhibitors
Xun Shang; Yi Zheng
29-38
Rational Design of Peptide Ligands Against a Glycolipid by NMR Studies
Wenyong Tong; Tara Sprules; Kalle Gehring; H. Uri Saragovi
39-52
A Combinatorial Strategy for the Acquisition of Potent and Specific Protein Tyrosine Phosphatase Inhibitors
Sheng Zhang; Lan Chen; David S. Lawrence; Zhong-Yin Zhang
53-65
Identification of Allosteric Inhibitors of p21-Activated Kinase
Julien Viaud; Jeffrey R. Peterson
67-79
Using a Modified Yeast Two-Hybrid System to Screen for Chemical GEF Inhibitors
Anne Blangy; Philippe Fort
81-95
Random Mutagenesis of Peptide Aptamers as an Optimization Strategy for Inhibitor Screening
Nathalie Bouquier; Sylvie Fromont; Anne Debant; Susanne Schmidt
97-118
A Screening Strategy for Trapping the Inactive Conformer of a Dimeric Enzyme with a Small Molecule Inhibitor
Charles S. Craik; Tina Shahian
119-131
Use of a Fluorescent ATP Analog to Probe the Allosteric Conformational Change in the Active Site of the Protein Kinase PDK1
Valerie Hindie; Laura A. Lopez-Garcia; Ricardo M. Biondi
133-141
Affinity Purification of Protein Kinases that Adopt a Specific Inactive Conformation
Pratistha Ranjitkar; Dustin J. Maly
143-151
Determination of the Kinetics and Thermodynamics of Ligand Binding to a Specific Inactive Conformation in Protein Kinases
Sanjay B. Hari; Pratistha Ranjitkar; Dustin J. Maly
153-159
Purification and Specific Assays for Measuring APE-1 Endonuclease Activity
Adrian Esqueda; Mohammed Z. Mohammed; Srinivasan Madhusudan; Nouri Neamati
161-174
An In Vitro Screening to Identify Drug-Resistant Mutations for Target-Directed Chemotherapeutic Agents
Mohammad Azam
175-184
Utilizing AntagomiR (Antisense microRNA) to Knock Down microRNA in Murine Bone Marrow Cells
Chinavenmeni S. Velu; H. Leighton Grimes
185-195
Synthesis, Conjugation, and Labeling of Multifunctional pRNA Nanoparticles for Specific Delivery of siRNA, Drugs, and Other Therapeutics to Target Cells
Peixuan Guo; Yi Shu; Daniel Binzel; Mathieu Cinier
197-219
Mouse Models for Tumor Metastasis
Shengyu Yang; J. Jillian Zhang; Xin-Yun Huang
221-228
Back Matter
229-230
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 19 of 19 entries
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