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Home > Synthetic Metabolic Pathways: Methods and Protocols > Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR
Synthetic Metabolic Pathways: Methods and Protocols |
Michael Krogh Jensen; Jay D. Keasling
Springer New York
Synthetic Metabolic Pathways: Methods and Protocols
Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR
Authors

Tadas Jakočiūnas; Emil D. Jensen; Michael K. Jensen; Jay D. Keasling

ARI Id

1665089757610_4782359

Access

Not Available Free

Pages

185-201

DOI

10.1007/978-1-4939-7295-1_12

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-4939-7295-1_12

Table of Contents of Book
Showing 1 to 20 of 27 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xii
Front Matter
1-1
Parts Characterization for Tunable Protein Expression
Michael S. Klausen; Morten O. A. Sommer
3-14
Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design
Jackson K. B. Cahn; Sabine Brinkmann-Chen; Frances H. Arnold
15-26
Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability
Ida Lauritsen; Virginia Martínez; Carlotta Ronda; Alex Toftgaard Nielsen; Morten H. H. Nørholm
27-37
An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization
Sean M. Halper; Daniel P. Cetnar; Howard M. Salis
39-61
Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design
Mohammad Mazharul Islam; Rajib Saha
63-82
Extended Metabolic Space Modeling
Pablo Carbonell; Baudoin Delépine; Jean-Loup Faulon
83-96
Computational Methods to Assess the Production Potential of Bio-Based Chemicals
Miguel A. Campodonico; Sumesh Sukumara; Adam M. Feist; Markus J. Herrgård
97-116
Front Matter
117-117
Multiplex Genome Editing in Escherichia coli
Sheila Ingemann Jensen; Alex Toftgaard Nielsen
119-129
Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-Gene Systems
Szu-Yi Hsu; Michael J. Smanski
131-147
An Adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism
Tian Zhang; Pier-Luc Tremblay
149-161
CRISPR-Cas9 Toolkit for Actinomycete Genome Editing
Yaojun Tong; Helene Lunde Robertsen; Kai Blin; Tilmann Weber; Sang Yup Lee
163-184
Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast Using CasEMBLR
Tadas Jakočiūnas; Emil D. Jensen; Michael K. Jensen; Jay D. Keasling
185-201
A Modified Gibson Assembly Method for Cloning Large DNA Fragments with High GC Contents
Lei Li; Weihong Jiang; Yinhua Lu
203-209
Coupling Yeast Golden Gate and VEGAS for Efficient Assembly of the Violacein Pathway in Saccharomyces cerevisiae
James Chuang; Jef D. Boeke; Leslie A. Mitchell
211-225
Front Matter
227-227
Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites for Phenotyping of Microorganisms
Christoph Halbfeld; Jörg Ingo Baumbach; Lars M. Blank; Birgitta E. Ebert
229-258
Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers
Maja Rennig; Daniel O. Daley; Morten H. H. Nørholm
259-268
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 27 entries
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