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Home > Protein Misfolding Diseases: Methods and Protocols > Methods to Screen Compounds Against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics
Protein Misfolding Diseases: Methods and Protocols |
Cláudio M. Gomes
Springer New York
Protein Misfolding Diseases: Methods and Protocols
Methods to Screen Compounds Against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics
Authors

Giulia Diniz da Silva Ferretti; Danielly C. Ferraz da Costa; Jerson L. Silva; Luciana Pereira Rangel

ARI Id

1665148803669_5042834

Access

Not Available Free

Pages

265-277

DOI

10.1007/978-1-4939-8820-4_17

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-4939-8820-4_17

Table of Contents of Book
Showing 1 to 20 of 26 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xiii
Front Matter
1-1
Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation
Joana S. Cristóvão; Bárbara J. Henriques; Cláudio M. Gomes
3-18
Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates
Eri Saijo; Bradley R. Groveman; Allison Kraus; Michael Metrick; Christina D. Orrù; Andrew G. Hughson et al.
19-37
Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides
Ravit Malishev; Sofiya Kolusheva; Raz Jelinek
39-51
Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis
Naoto Soya; Ariel Roldan; Gergely L. Lukacs
53-67
A Multipronged Method for Unveiling Subtle Structural–Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies
Donatella Bulone; Pier Luigi San Biagio; Tatiana Quiñones-Ruiz; Manuel Rosario-Alomar; Igor K. Lednev; Frank T. Robb et al.
69-92
High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1
Alireza Abdolvahabi; Sanaz Rasouli; Corbin M. Croom; Devon L. Plewman
93-108
Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases
Devkee M. Vadukul; Youssra K. Al-Hilaly; Louise C. Serpell
109-122
Assays for Light Chain Amyloidosis Formation and Cytotoxicity
Luis M. Blancas-Mejia; Pinaki Misra; Christopher J. Dick; Marta Marin-Argany; Keely R. Redhage; Shawna A. Cooper et al.
123-153
Front Matter
155-155
Monitoring Aggregate Clearance and Formation in Cell-Based Assays
Evelien Eenjes; Young Joo Yang-Klingler; Ai Yamamoto
157-169
Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor
Yu Liu; Matthew Fares; Xin Zhang
171-182
Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation
Vibha Prasad; Aaron Voigt
183-193
Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry
Salvador Ventura; Susanna Navarro
195-212
Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells
Edward Pokrishevsky; Jeremy Nan; Neil R. Cashman
213-224
A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction
Cagla Cömert; Paula Fernandez-Guerra; Peter Bross
225-239
Superresolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells
Steffen J. Sahl; Willianne I. M. Vonk
241-251
Front Matter
253-253
Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry
Tânia G. Lucas; Cláudio M. Gomes; Bárbara J. Henriques
255-264
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 26 entries
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