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Home > Post-Transcriptional Gene Regulation > A Step-by-Step Procedure to Analyze the Efficacy of siRNA Using Real-Time PCR
Post-Transcriptional Gene Regulation |
Jeffrey Wilusz
Humana Press
Post-Transcriptional Gene Regulation
A Step-by-Step Procedure to Analyze the Efficacy of siRNA Using Real-Time PCR
Authors

Angie Cheng; Charles L. Johnson; Lance P. Ford

ARI Id

1674219680723_2249976

Access

Open/Free Access

Pages

303-316

DOI

10.1007/978-1-59745-033-1_21

Chapter URL

https://rd.springer.com/protocol/10.1007/978-1-59745-033-1_21

Table of Contents of Book
Showing 1 to 20 of 26 entries
Chapters/HeadingsAuthor(s)PagesInfo
Front Matter
i-xviii
Front Matter
1-1
Bioinformatics Approaches for Studying Untranslated Regions of mRNAs
Paramjeet S. Bagga
1-21
Identification of mRNA Polyadenylation Sites in Genomes Using cDNA Sequences, Expressed Sequence Tags, and Trace
Ju Youn Lee; Ji Yeon Park; Bin Tian
23-37
Bioinformatic Tools for Studying Post-Transcriptional Gene Regulation: The UAlbany TUTR Collection and Other Informatic Resources
Francis Doyle; Christopher Zaleski; Ajish D. George; Erin K. Stenson; Adele Ricciardi; Scott A. Tenenbaum
39-52
Front Matter
51-51
In-Line Probing Analysis of Riboswitches
Elizabeth E. Regulski; Ronald R. Breaker
53-67
Ribotrap: Targeted Purification of RNA-Specific RNPs from Cell Lysates Through Immunoaffinity Precipitation to Identify Regulatory Proteins and RNAs
Dale L. Beach; Jack D. Keene
69-91
Advances in RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray Profiling
Timothy E. Baroni; Sridar V. Chittur; Ajish D. George; Scott A. Tenenbaum
93-108
Biosensors for RNA Aptamers—Protein Interaction
Sara Tombelli; Maria Minunni; Marco Mascini
109-119
A Tethering Approach to Study Proteins that Activate mRNA Turnover in Human Cells
Sandra L. Clement; Jens Lykke-Andersen
121-133
RNA Analysis by Biosynthetic Tagging Using 4-Thiouracil and Uracil Phosphoribosyltransferase
Gusti M. Zeiner; Michael D. Cleary; Ashley E. Fouts; Christopher D. Meiring; Edward S. Mocarski; John C. Boothroyd
135-146
Efficient 5' Cap-Dependent RNA Purification: Use in Identifying and Studying Subsets of RNA
Edyta Z. Bajak; Curt H. Hagedorn
147-160
Front Matter
159-159
Enrichment of Alternatively Spliced Isoforms
Julian P. Venables
161-170
In Vivo Methods to Assess Polyadenylation Efficiency
Lisa K. Hague; Tyra Hall-Pogar; Carol S. Lutz
171-185
Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells
Roy M. Long; Carl R. Urbinati
187-196
Analysis of mRNA Partitioning Between the Cytosol and Endoplasmic Reticulum Compartments of Mammalian Cells
Samuel B. Stephens; Rebecca D. Dodd; Rachel S. Lerner; Brook M. Pyhtila; Christopher V. Nicchitta
197-214
In Vivo and In Vitro Analysis of Poly(A) Length Effects on mRNA Translation
Jing Peng; Elizabeth L. Murray; Daniel R. Schoenberg
215-230
A Ribosomal Density-Mapping Procedure to Explore Ribosome Positions Along Translating mRNAs
Naama Eldad; Yoav Arava
231-242
Chapters/HeadingsAuthor(s)PagesInfo
Showing 1 to 20 of 26 entries
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